This book serves as an excellent example of the value and advancements of the disease model for malaria, a widespread issue in public health. The biology of malaria in a liver and all in vitro platforms for liver-stage malaria are thoroughly covered in this book, along with their principles, protocols, applications for disease modeling and drug screening, and drawbacks. The basis for the formation of stem cells in the liver during development and in adults is described in the first chapter. The following chapters cover new and recent developments in simulating malaria using liver organoids and liver-on-a-chip. The book outlines current protocols and techniques for producing liver organoids and livers on chips, along with their benefits and drawbacks. The humanized mouse model of liver-stage malaria employing ectopic prosthetic livers is discussed in the book’s final section along with innovative readout modalities.
For evaluating the viability and functionality of hepatocyte formation, the recent developments and difficulties in liver-on-a-chip technology are highlighted. The book explains how these 3D models can be used to comprehend the biological complexity of the cellular and molecular pathways involved in Plasmodium development in the liver, as well as toolkits to examine parasite deployment in the 3D models and to implement in drug discovery. The final section of the book covers the obstacles and potential future prospects for liver organoids and liver on-chip applications in the biology of live-stage malaria. Researchers and scientists working in the fields of parasitology, cell biology, tissue engineering, and pharmacology will find this book useful.​
