The eighth edition of Freshney’s Culture of Animal Cells stands as the most comprehensive and contemporary guide to the fundamental methodology of cell culture. This indispensable resource has been fully updated to reflect the latest advancements and applications in the field, encompassing topics like iPSCs, CRISPR, and organ-on-chip technologies. It provides insights into both the “how” and “why” of tissue culture techniques, covering cell biology, media and substrate selection, regulatory considerations, laboratory procedures, aseptic techniques, and much more.
Key features of the eighth edition include:
Incorporation of Cutting-Edge Techniques: This edition has been thoroughly revised to encompass the most recent techniques and emerging applications in cell culture. It explores the utilization of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions in stem cell culture.
New Chapters: A new chapter delves into the origin and evolution of cell lines, while another addresses the issue of irreproducible research, its causes, and the critical importance of reproducibility and adhering to good cell culture practices.
Comprehensive Coverage: The book offers single-volume coverage of fundamental skills and protocols, specialized techniques and applications, as well as the latest developments in the field. It includes critical areas such as laboratory design, safety protocols, bioethics, media preparation, primary culture, mycoplasma testing, cell line characterization, cryopreservation, and more.
Updated Content: The eighth edition features updated chapters and protocols on various topics, including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing.
Online Resources: Readers can access supplementary resources on the companion website, including figures, artwork, and additional protocols for reference and printing.
Freshney’s Culture of Animal Cells continues to be an essential reference for a broad range of professionals, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic scientists, laboratory managers, technicians, and trainees working across cell biology, molecular biology, and genetics disciplines. It serves as a comprehensive guide for those involved in cell culture techniques and their applications.
